Check Similarity : Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells

Novilla, Arina and Somantri Djamhuri, Dedi and Nurhayati, Betty and Davidson Rihibiha, Dwi and Afifah, Ervi and Widowati, Wahyu (2017) Check Similarity : Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells. Asian Pacific Journal of Tropical Biomedicine, 7 (11). pp. 1005-1009. ISSN 2221-1691

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Abstract

Objective: To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7). Methods: A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells. Interleukins (IL-6, IL-1b), tumor necrosis factor-a (TNF-a), and cyclooxigenase-2 (COX-2) levels were measured by ELISA, and nitric oxide (NO) levels measured by a nitrate/nitrite colorimetric assay to determine the inhibition activity of OTEE and EGCG. Results: Lipopolysaccharide induction increases NO, COX-2, IL-6, IL-1b, and TNF-a levels compared with the untreated cell (negative control). The positive control, lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study. The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO, COX-2, IL-6, IL-1b, and TNF-a. OTEE had a higher inhibition activity toward NO, COX-2, IL-6, and IL-1b than EGCG; the reverse was seen for TNF-a. However, both OTEE and EGCG suppressed production of NO, COX-2, IL-6, IL-1b, and TNF-a. Conclusions: OTEE and EGCG have the potential for use as anti-inflammatory drugs, which is shown by their ability to reduce the production of NO, COX-2, IL-6, IL-1b, and TNF-a in active macrophages.

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