PERBANDINGAN PEMERIKSAAN KADAR HEMOGLOBIN DENGAN METODE SIANMETHEMOGLOBIN DAN SODIUM LAURYL SULPHATE HEMOGLOBIN

Sudrajat, Nabilla Zeinia and Durachim, Adang and Marliana, Nina and Nurhayati, Betty (2020) PERBANDINGAN PEMERIKSAAN KADAR HEMOGLOBIN DENGAN METODE SIANMETHEMOGLOBIN DAN SODIUM LAURYL SULPHATE HEMOGLOBIN. Diploma thesis, Politeknik Kesehatan Kemenkes Bandung.

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Abstract

ABSTRAK Pemeriksaan hemoglobin adalah suatu prosedur pemeriksaan yang sering dilakukan di laboratorium untuk mendeteksi dan memantau pengobatan anemia serta menggambarkan tingkat keparahannya. Pemeriksaan kadar hemoglobin dapat ditentukan dengan metode Sianmethemoglobin dan Sodium Lauryl Sulphate Hemoglobin. Metode sianmethemoglobin memiliki keunggulan yaitu ketersediaan referensi standar / kalibrator yang stabil dan diterima secara internasional, namun pembuangan sejumlah besar reagen Drabkin yang mengandung sianida dapat menambah potensi bahaya bio-toksik. Sedangkan metode Sodium Lauryl Sulphate Hemoglobin menggunakan reagen non-toksik tidak mengandung sianida. Sehingga penelitian dilakukan dengan tujuan untuk mengetahui apakah terdapat perbedaan hasil pemeriksaan kadar Hemoglobin dalam darah yang ditentukan dengan metode Sianmethemoglobin dan Sodium Lauryl Sulphate Hemoglobin. Penelitian ini menggunakan jenis penelitian deskriptif dengan desain tinjauan pustaka sistematis (studi literatur). Literatur yang digunakan dalam penelitian adalah lima jurnal penelitian yang berkaitan dengan perbandingan metode Sianmethemoglobin dan Sodium Lauryl Sulphate Hemoglobin. Waktu penelitian dilaksanakan pada bulan Juni-Juli 2020. Pengumpulan data dilakukan dengan metode observasi terhadap jurnal-jurnal penelitian terkait dan data penelitian dikumpulkan, diolah, disajikan, serta diinterpretasikan. Hasil penelitian didapatkan bahwa kadar Hemoglobin yang diukur menggunakan metode Sodium Lauryl Sulphate Hemoglobin serupa dengan metode Sianmethemoglobin dan memiliki koefisien korelasi yang baik berkisar pada 0,99. Sehingga, kesimpulan penelitian ini adalah tidak terdapat perbedaan hasil pemeriksaan kadar Hemoglobin dalam darah yang ditentukan dengan metode Sianmethemoglobin dan Sodium Lauryl Sulphate Hemoglobin. Kata kunci: Kadar Hemoglobin, metode Sianmethemoglobin, metode Sodium Lauryl Sulphate Hemoglobin ABSTRACT Haemoglobin estimation is one of the most common tests which are done at laboratories for detect and monitor treatment of anemia as well as describe its severity. Hemoglobin concentration can estimed by Cyanmethemoglobin method and Sodium Lauryl Sulphate Hemoglobin method. Cyanmethemoglobin method its advantage is the ready availability of a stable and internationally accepted reference standard/calibrator, however as the disposal of large volumes of Drabkin reagent which contains cyanide may constitute a potential bio-toxic hazard. While Sodium Lauryl Sulphate Hemoglobin method using non-toxic reagents does not use cyanide. The study was conducted with aims to find out whether there are differences in the results of Hemoglobin concentration in the blood by using Cyanmethemoglobin method and Sodium Lauryl Sulphate Hemoglobin method. This research uses descriptive research with systematic literature review (literature study). Literatures are used in the research is five research journals that related to comparison of Cyanmethemoglobin method and Sodium Lauryl Sulphate Hemoglobin method. The time of the study was conducted in June-July 2020. Data collection is done by observation method on research journals and the research data collected, processed, presented, and interpreted. the results of the study show that the haemoglobin concentrations which were estimated by the Sodium Lauryl Sulphate method were similar to those which were obtained by the Cyanmethaemoglobin method and good correlation coefficienty is arround 0,99. The conclusion of this study is that there is no difference in the results of Hemoglobin concentration in the blood which were estimated by Cyanmethemoglobin method and Sodium Lauryl Sulphate Hemoglobin method. Keyword: Hemoglobin concentration, Cyanmethemoglobin method, Sodium Lauryl Sulphate Hemoglobin method

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