PENGARUH VARIASI SUHU AWAL REAGEN TERHADAP HASIL PEMERIKSAAN GLUKOSA METODE GOD-PAP

Safitri, Rizka Amelia and Nurhayati, Dewi and Merdekawati, Fusvita and Kurnaeni, Nani (2021) PENGARUH VARIASI SUHU AWAL REAGEN TERHADAP HASIL PEMERIKSAAN GLUKOSA METODE GOD-PAP. Diploma thesis, Politeknik Kesehatan Kemenkes Bandung.

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Abstract

THE EFFECT OF VARIATIONS OF INITIAL REAGENTS TEMPERATURES ON THE RESULTS OF THE GOD-PAP METHOD OF Glucose Examination ABSTRACT Blood glucose is one of the parameters of clinical chemistry examination in the laboratory for supporting examinations, one of which uses the enzymatic method. One of the factors that affect enzyme activity is temperature. Based on the GOD-PAP reagent kit, it must be stored at refrigerator temperature and when used for blood glucose examination, the reagent must be stored at room temperature. The purpose of this study was to determine the effect of variations in the initial temperature of the reagent on blood glucose levels by the enzymatic method. This type of research is experimental. The sample used the serum of a D III student, Health Analyst, Poltekkes Ministry of Health, Bandung class 3B, Class of 2019 which was examined at the initial temperature of the refrigerator reagent, a temperature of 25˚C and a temperature of 37˚C. The data obtained were analyzed statistically with the Shapiro Wilk test, the data were normally distributed and the One Way Anova test was performed. The results of the study with the initial temperature of the Refrigerator reagent the average value of glucose levels is 84 mg/dL, at the initial temperature of the reagent 25˚C the average value of glucose levels is 111.75 mg/dl, while the initial temperature of the reagent 37C is 89 mg /dl. One Way Anova test obtained a significance value = 0.000 then Ho is rejected and Ha is accepted, so it can be concluded that there is a significant effect of variations in the initial temperature of the reagents on the results of blood glucose levels examined at the initial temperature of the reagent 8 ˚C , the initial temperature of the reagent 25˚C and at the initial temperature of the reagent. 37˚C. Keywords: GOD-PAP Reagent, Blood Glucose, Temperature Variations

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