ANTISEPTIC EFFECTIVENESS OF GUAVA LEAVE EXTRACT (Psidium guajava L.), TURMERIC EXTRACT (Curcuma longa L.) AND BIOPLACENTON AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

Kurniati1, Iis and Marliana1, Nina and Triana2, Linda and Dermawan1, Asep and Rudiansyah1, Deny and Ilmi Sufa1, Hafizah (2021) ANTISEPTIC EFFECTIVENESS OF GUAVA LEAVE EXTRACT (Psidium guajava L.), TURMERIC EXTRACT (Curcuma longa L.) AND BIOPLACENTON AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA). Proceeding of the 4th International Conference on Interprofessional Health Collaboration and Community Empowerment, - (-). pp. 157-162. ISSN 2809-5316

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Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) against various antimicrobials, especially based on the insertion of mobile genetic elements, is called staphylococcal cassette chromosome mec (SCCmec) on chromosome Staphylococcus aureus. Antiseptic is an anti-bacterial that fights pathological flora mechanically, chemically, or a combination of both, with the aim of killing, inhibiting, or reducing the number of microorganisms such as Staphylococcus aureus. Antibiotic resistance has been one of the major public health problems humans face since the discovery of antibiotics. S. aureus has been resistant to methicillin and other -lactam antibiotics such as penicillin, cephalosporin, monobactam, and carbapenem. This group of S. aureus is known as Methicillin-Resistant Staphylococcus aureus (MRSA). Infections caused by MRSA have spread rapidly and are found in almost all countries. Guava leaves (Psidium guajava L) and turmeric (Curcuma longa L) are plants in journals showing therapeutic effects such as anti-inflammatory, antioxidant, antiviral, anti-bacterial, and antifungal activities and other activities. This research was conducted to determine whether guava leaf extract and turmeric can be used as an anti-bacterial against MRSA and the minimum guava and turmeric leaf extract concentration, effectively inhibiting MRSA. Methods: this study used guava leaf extract 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% and 20% respectively and turmeric 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% and 10%. Bioplacenton is used as a control which is commonly used as an antiseptic. The design of this study used an experimental laboratory design. The treatment group (dilution of guava leaf extract and turmeric) is contacted against MRSA, equivalent to the standard Mc Farland 0.5. MRSA planted in the media Mueller Hinton Agar (MHA) were incubated at 37 ° C for 24 hours in the hole method. Conclusions: the results of the observations were analyzed using the ANOVA test. The minimum concentration of guava leaf extract (Psidium Guajava L), effective against MRSA, is 18%. In comparison, turmeric (Curcuma longa L) is 10%, controlling bioplacenton inhibition of 15 mm in diameter. Keywords: Antiseptic, Psidium guajava L., Curcuma longa L., Bioplacenton, MRSA (Methicillin-Resistant Staphylococcus aureus)

Item Type: Article
Subjects: 600 Teknologi dan Ilmu-ilmu Terapan > 610 - 619 Ilmu Kedokteran dan Ilmu Pengobatan > 610 Ilmu Kedokteran, Ilmu Pengobatan dan Ilmu Kesehatan
600 Teknologi dan Ilmu-ilmu Terapan > 610 - 619 Ilmu Kedokteran dan Ilmu Pengobatan
Divisions: Jurusan Teknik Laboratorium Medik > D IV
Depositing User: Mrs Nina Marliana
Date Deposited: 31 Mar 2023 02:52
Last Modified: 31 Mar 2023 02:52
URI: http://repo.poltekkesbandung.ac.id/id/eprint/6794

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